Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay.

Despite the emergence of the pandemic H1N1 influenza A virus in 2009, seasonal H3N2 viruses continue to co-circulate in the population and may even predominate in the coming influenza season. We describe a specific minor groove binder TaqMan assay for H3N2 viruses with a detection limit of 16.5 standard DNA copies.

متن کامل

Real-time PCR-based pathotyping of Newcastle disease virus by use of TaqMan minor groove binder probes.

A real-time reverse-transcription PCR was developed to detect and pathotype Newcastle disease viruses (NDV) in clinical samples. Degenerate oligonucleotide primers and TaqMan probes with nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F) gene that corresponds to the cleavage site of the F0 precursor, which is a key determinant of NDV...

متن کامل

Rapid detection of the factor V Leiden mutation by real-time PCR with TaqMan minor groove binder probes.

Troponin-tropomyosin interactions. Fluorescence studies of the binding of troponin, troponin T, and chymotryptic tropo-nin T fragments to specifically labeled tropomy-osin. To the Editor: Individuals heterozygous for the factor V Leiden mutation have a seven-fold higher risk to develop venous thrombosis than wild-type (WT) individuals (1). The allele frequency of factor V Leiden in Europeans is...

متن کامل

Development of a TaqMan minor groove binding protein assay for the detection and quantification of Crimean-Congo hemorrhagic fever virus.

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus of the genus Nairovirus and the family Bunyaviridae. It is a negative-strand RNA virus comprised of small (S), medium (M), and large (L) genome segments. The S segment encodes for nucleocapsid protein, the M segment codes for envelope glycoproteins (Gn and Gc), and the L segment codes for the RNA-dependent RNA polymerase. Curre...

متن کامل

Detection of Seasonal Influenza H1N1 and H3N2 Viruses using RT-PCR Assay during 2009 Flu Pandemic in Golestan Province

Abstract Background and Objective: The emergence of a novel H1N1influenza A virus of animal origin with transmissibility from human to human poses pandemic concern. Current subtypes of Seasonal influenza A viruses spread in human are influenza A H1N1 influenza A H3N2 and influenza type B viruses. The aim of this study was to determine current strains of the H3N2 and new H1N1 subtypes of influe...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Diagnostic Microbiology and Infectious Disease

سال: 2011

ISSN: 0732-8893

DOI: 10.1016/j.diagmicrobio.2011.01.012